Measure Protein A205; Measure Protein Concentration At A205 - Thermo Scientific NanoDrop One User Manual

5 Protein Applications

Measure Protein A205

Measure Protein A205

Measure Protein Concentration at A205

To measure Protein A205 samples
78 NanoDrop One User Guide
Measures the concentration of purified
protein populations that absorb at
205 nm.
Measure A205 Proteins
Reported Results
Settings
Detection Limits
Calculations
Use the Protein A205 application to quantify purified peptides and other proteins that
contain peptide bonds, which exhibit absorbance at 205 nm. This application
reports protein concentration and two absorbance values (A205 and A280). A
single-point baseline correction can also be used. This application does not require a
standard curve.
Note If your samples contain mainly amino acids such as tryptophan or
tyrosine, or cys-cys disulfide bonds, use the
Protein A205.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
Protein A280 application instead of
Cleaning the
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