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5
Protein Applications
Measure Proteins and Labels
Setting
Available Options
Sample
1 Abs = 1 mg/mL
a
type
BSA
IgG
Lysozyme
Other protein
(
+ MW)
Other protein
(
1%)
Analysis
On or off
b
Correction
Enter analysis
correction wavelength
in nm or use default
value (340 nm)
Dye 1/Dye 2
Cy3, 5, 3.5, or 5.5,
c
Type
Alexa Fluor 488, 546,
555, 594, 647, or 660
Dye 1/Dye 2
picomoles/microliter
Unit
(pmol/uL), micromoles
(uM), or millimoles
(mM)
90
NanoDrop One User Guide
Mass Ext.
Coefficient
Description
(L/gm-cm)
General reference
Tap here
available setting.
6.7
Each sample type applies a unique extinction
13.7
coefficient to the protein calculations. If the
extinction coefficient of the sample is known,
26.4
choose the
option and enter the value. Otherwise,
user-entered molar
calculate the extinction coefficient or choose
extinction
the option that best matches the sample
coefficient/
solution. If you only need a rough estimate of
molecular weight
protein concentration and the sample
User entered mass
extinction coefficient is unknown, select the
extinction
1 Abs=1 mg/mL sample type option.
coefficient
Tip: Ideally, the extinction coefficient should
be determined empirically using a solution of
the study protein at a known concentration
using the same buffer.
N/A
Corrects sample absorbance measurement
for any offset caused by light scattering
particulates by subtracting absorbance value
at specified analysis correction wavelength
from absorbance value at analysis
wavelength. Corrected value is used to
calculate sample concentration.
Tip: If the sample has a modification that
absorbs light at 340 nm, select a different
correction wavelength or turn off Analysis
Correction.
See
Select pre-defined dye used to label sample
Dye/Chromophore
material, or one that has been added using
Editor
for specific
Dye/Chrom. Editor.
values for each
dye
not applicable
Select unit for reporting dye concentrations.
for detailed description of each
+ MW (molar) or
Thermo Scientific
1% (mass)
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