Measure Uv-Vis - Thermo Scientific NanoDrop One User Manual

Micro-uv/vis spectrophotometer
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7
Custom Applications

Measure UV-Vis

Measure UV-Vis
Measure UV-Vis
To make UV-Vis measurements
146
NanoDrop One User Guide
Measures the absorbance of any
sample at up to 40 wavelengths
across the ultra-violet (UV) and visible
regions of the spectrum.
Measure UV-Vis
Reported Results
Settings
Detection Limits
The UV-Vis application allows the instrument to function as a conventional
spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to
850 nm. Up to 40 wavelengths can be designated for absorbance monitoring and
inclusion in the report. Automatic pathlength adjustment and a single-point baseline
correction can also be used.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure a sample using the UV-Vis application
1. From the Home screen, from the Custom tab, select UV-Vis.
2. Specify up to
40 wavelengths to monitor
and whether automated pathlength adjustment, analysis wavelength, and
baseline correction will be used.
Cleaning the
(or you can specify them later if desired)
Thermo Scientific

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