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7
Custom Applications
Measure Custom
Setting
Analysis wavelength
(Standard curve methods
only)
Baseline correction
164
NanoDrop One User Guide
Available Options
• Select curve fit type.
Specify type of equation used to create standard curve from standard
concentration values. Available options:
–
Linear: Draws the linear least squares line through all measured
standards (requires reference measurement and at least one
standard)
–
Interpolation: Draws a series of straight lines to connect all measured
standards (requires reference measurement ans at least one
standard)
nd
–
2
order polynomial: Draws the 2
using all measured standards (requires reference measurement and at
least standards)
rd
–
3
order polynomial: Draws the 3
using all measured standards (requires reference measurement and at
least three standards)
Monitor absorbance at specified wavelength (enter the wavelength in nano-
meters).
Note: The specified wavelength must fall within the selected
range.
The measurement results or the concentration will be calculated automatically
using the absorbance value at the specified wavelength and applying the
selected method type (factor or standard curve).
Select this option to correct offset caused by light scattering particulates by
subtracting the absorbance at a specified baseline point. Then specify
wavelength for baseline correction.
Note: Software subtracts absorbance value at specified baseline correction
wavelength from absorbance values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is zero at specified baseline
correction wavelength.
nd
order least squares polynomial
rd
order least squares polynomial
measurement
Thermo Scientific
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