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5
Protein Applications
Measure Proteins and Labels
Calculations for Proteins and Labels Measurements
As with the other protein applications,
Proteins & Labels uses the
equation
to correlate absorbance with
concentration based on the sample's
extinction coefficient and pathlength.
This application offers six options (shown at
right) for selecting an appropriate extinction
coefficient for each measured sample, to
be used in conjunction with Beer's Law to
calculate sample concentration.
If the extinction coefficient of the sample is
known, choose the
+ MW (molar) or
1% (mass) option and enter the value.
Otherwise, calculate the extinction
coefficient or choose the option that best
matches the sample solution.
Tip: Ideally, the extinction coefficient
should be determined empirically using a
solution of the study protein at a known
concentration using the same buffer.
92
NanoDrop One User Guide
Available Options for Extinction Coefficient
Beer-Lambert
• 1 Abs = 1 mg/mL, where sample type and/or ext.
• BSA (Bovine Serum Albumin, 6.7 L/gm-cm)
• IgG (any mammalian antibody, 13.7 L/gm-cm)
• Lysozyme (egg white lysozyme, 26.4 L/gm-cm)
• Other protein (
• Other protein (
Note: See
coefficient is unknown (produces rough estimate of
protein concentration)
+ MW), user-specified molar ext.
coefficient
1%), user-specified mass ext.
coefficient
Sample Type
for details.
Thermo Scientific
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