Thermo Scientific NanoDrop One User Manual page 143

Micro-uv/vis spectrophotometer
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Setting
Absorbance
correction
Thermo Scientific
Available Options
Absorbance value
between 0 and 300 A
Description
User-defined absorbance correction. Enter absorbance
correction for displayed spectrum. This can be useful, for
example, to correct baseline offset caused by any
difference between the media solution used to blank the
instrument and media used to suspend the cell culture
sample, and because scattered light generally produces
an offset.
Absorbance correction value is subtracted from
absorbance values at all wavelengths in sample spectrum.
(All displayed absorbance values are corrected values.)
6
Measure OD600
NanoDrop One User Guide
141

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