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Settings for UV-Vis Measurements
Setting
Available Options
Monitored
Enter up to 40
wavelengths
wavelengths between
190 nm and 850 nm
Analytical
Any wavelength between
Wavelength
190 nm and 850 nm
Automated
On or Off
Pathlength
(affects pedestal
measurements only)
Thermo Scientific
To show the UV-Vis settings, from the Home screen, from the Custom tab, select
UV-Vis.
Description
User-defined wavelengths to be measured and
reported at run time. Absorbance values for the first
three entered wavelengths are displayed in the
measurement
screen. To see absorbance values for 8
monitored wavelengths, swipe left in the measurement
screen to show the
Data
wavelengths, press and hold a sample row to show the
Sample Details
screen (scroll up to display absorbance
values for any additional user-defined wavelengths).
Note: If Baseline Correction is selected, all displayed
absorbance values are the corrected values.
This is the wavelength the software will use to determine
the pathlength selection.
Optional automated pathlength selection. Allows
the software to use the optimal (shorter) pedestal
pathlength for high concentration samples to help prevent
detector saturation (see
• When selected, the shorter pathlength is used when
any wavelength between 220 nm and 850 nm has
10 mm equivalent absorbance value of 12.5 or higher.
For wavelengths between 190 nm and 219 nm the
change to the shorter pathlength occurs when any
wavelength in this range has a 10 mm equivalent
absorbance value of 10 or higher.
• When deselected, the pedestal pathlength is restricted
to 10 mm across all wavelengths.
Note: In either case, displayed absorbance values have
been normalized to a 1 mm pathlength equivalent.
7
Custom Applications
Measure UV-Vis
table. To see all monitored
Detection Limits
for details).
NanoDrop One User Guide
151
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