Table of Contents
Advertisement
To measure Protein BCA standards and samples
Thermo Scientific
• After the minimum number of standards has been measured for the selected
curve type, the message "Invalid Curve" changes to "Valid Curve." (This occurs
even when additional standards have been defined but not yet measured.) If the
"Invalid Curve" message remains after all entered standards have been
measured, try:
–
selecting a different curve type
–
remeasuring standards using the correct standard material
Valid Curve indicator: This is only an indicator that the required minimum
number of points has been established for the selected curve type. It does not
validate the integrity of the curve. For example, additional standards may be
required to cover the expected assay concentration range.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure Protein BCA standards and samples
1. From the Home screen, select the Proteins tab and tap Protein BCA.
2. Specify a
curve type
concentration of each
Tip: For this assay, we recommend setting Curve Type to "Linear".
3. Measure blank:
–
pipette 2 µL DI H
blanking cuvette into cuvette holder
and number of
replicates for each standard
standard.
O onto lower pedestal and lower arm, or insert DI H
2
5
Protein Applications
Measure Protein BCA
Cleaning the
and enter the
NanoDrop One User Guide
O
2
101
Advertisement
Table of Contents
