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Best practices for nucleic acid measurements
Thermo Scientific
Typical nucleic acid spectrum
Comparison of nucleic acid spectra with and without two
common contaminants
• Isolate and purify nucleic acid samples before measurement to remove
impurities. Depending on the sample, impurities could include DNA, RNA, free
nucleotides, proteins, some buffer components and dyes. See
Samples
for more information.
Note Extraction reagents such as guanidine, phenol, and EDTA contribute
absorbance between 230 nm and 280 nm and will affect measurement
results if present in samples (even residual amounts).
4
Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
Preparing
NanoDrop One User Guide
25
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