Measure Using A Custom Factor; Measure Nucleic Acid Using A Custom Factor - Thermo Scientific NanoDrop One User Manual

Measure using a Custom Factor

Measure Nucleic Acid using a Custom Factor

To measure nucleic acid samples using a custom factor
Thermo Scientific
Measures the concentration of
purified nucleic acids using a
custom factor for the
calculations.
Measure using Custom Factor
Reported Results
Settings
Detection Limits
Calculations
Use the Custom Factor application to quantify purified DNA or RNA samples that
absorb at 260 nm with a user-defined extinction coefficient or factor. The application
reports nucleic acid concentration and two absorbance ratios (A260/A280 and
A260/A230). A single-point baseline correction can also be used.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure using a custom factor
1. From the Home screen, select the Nucleic Acids tab and select Custom
Factor.
4
Nucleic Acid Applications
Measure using a Custom Factor
Cleaning the
NanoDrop One User Guide 45