Table of Contents
Advertisement
Measure Protein Bradford
Measure Total Protein Concentration
Theory of Protein Bradford assay
Thermo Scientific
Measures total protein concentration of
unpurified protein samples using a
Coomassie Blue dye colorimetric
detection reagent.
Measure Total Protein
Reported Results
Settings
Detection Limits
The Protein Bradford assay uses Coomassie Blue dye as a colorimetric detection
reagent to determine total protein concentration in unpurified protein samples. This
application is useful for measuring dilute protein solutions that require lower
detection sensitivity or proteins in the presence of components that exhibit
significant absorbance between 200 nm and 280 nm, which rules out direct protein
measurements at 280 nm or 205 nm. This application measures absorbance at
595 nm and uses a standard curve to calculate protein concentration. See
with Standard Curves
applied.
The Protein Bradford assay uses the protein-induced absorbance shift of Coomassie
Blue dye to determine total protein concentration. The bound protein-dye complex is
measured at 595 nm and baseline-corrected using the absorbance value at 750 nm.
Pre-formulated kits of stabilized reagent mixture containing Coomassie Blue dye,
alcohol, and surfactant are available from us or a local distributor.
To maximize reliability with the Protein Bradford assay:
• Work quickly and do not allow prepared standards or samples to
sit longer than necessary. Coomassie dye-dye and Coomassie dye-protein
aggregates can form particulates with increasing development time, resulting in
significant fluctuations in absorbance readings.
for more information. A single-point baseline correction is
5
Protein Applications
Measure Protein Bradford
NanoDrop One User Guide
Working
109
Advertisement
Table of Contents
