Measure Oligo Dna Or Oligo Rna - Thermo Scientific NanoDrop One User Manual

Measure Oligo DNA or Oligo RNA

Measure Oligo DNA or Oligo RNA
To measure Oligo DNA or Oligo RNA samples
Thermo Scientific
Measures the concentration of purified
ssDNA or RNA oligonucleotides that
absorb at 260 nm.
Measure Oligo DNA or RNA
Reported Results
Settings
Detection Limits
Calculations
Use the Oligo DNA and Oligo RNA applications to quantify oligonucleotides that
absorb at 260 nm. Molar extinction coefficients are calculated automatically based
on the user-defined base sequence of the sample. These applications report nucleic
acid concentration and two absorbance ratios (A260/A280 and A260/A230). A
single-point baseline correction can also be used.
Note If the oligonucleotide has been modified, for example with a fluorophore
dye, check with the oligo manufacturer to determine if the modification
contributes absorbance at 260 nm. If it does, we recommend using the
Microarray application to quantify nucleic acid concentration. The Microarray
application includes a correction to remove any absorbance contribution due to
the dye from the oligo quantification result.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
4
Nucleic Acid Applications
Measure Oligo DNA or Oligo RNA
Cleaning the
NanoDrop One User Guide 51