Detection Limits For Proteins And Labels Measurements - Thermo Scientific NanoDrop One User Manual

Setting Available Options
Sloping Dye On or off
d
Correction
a
To add or edit a custom protein, use
b
Analysis Correction affects calculation for protein concentration only.
c
To add custom dye or edit list of available dyes, use
d
Sloping Dye Correction affects calculations for dye concentration only.

Detection Limits for Proteins and Labels Measurements

To calculate upper detection limits for other (non-BSA) protein sample types
Thermo Scientific
Mass Ext.
Coefficient
(L/gm-cm)
Protein Editor.
Dye/Chromophore
Related Topics
• Instrument Settings
• Protein Editor
• Dye/Chromophore Editor
Detection limits and reproducibility specifications for purified BSA proteins and dyes
that are pre-defined in the software are provided here. The BSA lower detection limit
and reproducibility values apply to any protein sample type. The upper detection
limits are dependent on the
sample's extinction coefficient.
To calculate upper detection limits in mg/mL for proteins, use the following equation:
(upper absorbance limit
For example, if the sample's mass extinction coefficient at 280 nm is 6.7 for a 1%
(10 mg/mL) solution, the equation looks like this:
(550 / 6.7) * 10 = 824.6 (or ~825)
Related Topics
• Detection Limits for All Applications
Description
Corrects dye absorbance measurements for
any offset caused by light scattering
particulates by subtracting absorbance value
of a sloping baseline from 400 nm to 750 nm
from absorbance value at dye's analysis
wavelength.
Editor.
upper absorbance limit
/mass extinction coefficient
instrument
5
Protein Applications
Measure Proteins and Labels
of the instrument and the
) * 10
sample
NanoDrop One User Guide 91