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5
Protein Applications
Measure Protein A280
Detection Limits for Protein A280 Measurements
To calculate upper detection limits for other (non-BSA) protein sample types
72
NanoDrop One User Guide
Edit custom protein
1. In Protein Editor, tap to select custom protein
2. Tap
to show the Edit Protein Type box
3. Edit any entries or settings
4. Tap OK
Delete custom protein
1. In Protein Editor, tap to select a custom protein to delete
2. Tap
Note Deleting a custom protein permanently removes the protein and all
associated information from the software.
Detection limits and reproducibility specifications for purified BSA proteins are
provided here. The BSA lower detection limit and reproducibility values apply to any
protein sample type. The upper detection limits are dependent on the
absorbance limit
of the instrument and the sample's extinction coefficient.
To calculate upper detection limits in ng/µL for proteins, use the following equation:
(upper absorbance limit
For example, if the sample's mass extinction coefficient at 280 nm is 6.7 for a 1%
(10 mg/mL) solution, the equation looks like this:
(550 / 6.7) * 10 = 824.6 (or ~825)
/mass extinction coefficient
t
instrumen
upper
) * 10
sample
Thermo Scientific
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