Calculations For Microarray Measurements - Thermo Scientific NanoDrop One User Manual

Calculations for Microarray Measurements

As with the other nucleic acid applications,
the Microarray application uses a
modification of the Beer-Lambert equation
to calculate sample concentration where
the extinction coefficient and pathlength
are combined and referred to as a "factor."
The Microarray application offers six
options (shown at right) for selecting an
appropriate factor for each measured
sample, to be used in conjunction with
Beer's Law to calculate sample
concentration.
If the factor is known, choose the Custom
Factor option and enter the factor in
ng-cm/µL. Otherwise, choose the option
that best matches the sample solution.
Tip: Ideally, the factor or extinction
coefficient should be determined
empirically using a solution of the study
nucleic acid at a known concentration
using the same buffer.
Thermo Scientific
Available Options for Factors
• dsDNA (factor = 50 ng-cm/µL)
• ssDNA (factor = 33 ng-cm/µL)
• RNA (factor = 40 ng-cm/µL)
• Oligo DNA (calculated from user entered DNA
nucleotide sequence)
• Oligo RNA (calculated from user entered RNA
nucleotide sequence)
• Custom Factor (user entered factor between
15 ng-cm/µL and 150 ng-cm/µL
Note: See Sample Type
4
Nucleic Acid Applications
Measure Microarray
for more information.
NanoDrop One User Guide 41