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Detection Limits for Nucleic Acid Measurements using a Custom
Factor
To calculate upper detection limits for nucleic acid samples
Thermo Scientific
The lower detection limits and reproducibility specifications for nucleic acids are
provided here. The upper detection limits are dependent on the
limit
of the instrument and the user-defined extinction coefficients.
To calculate upper detection limits in ng/µL, use the following equation:
(upper absorbance limit
For example, for a sample measurement using an extinction coefficient of 55, the
equation looks like this:
(550 AU * 55 ng-cm/µL) = 30,250 ng/µL
Note For measurements with 10 mm pathlength cuvettes, the upper
absorbance limit is 1.5 AU, which is approximately 75 ng/µL for dsDNA.
Related Topics
•
Detection Limits for All Applications
* extinction coefficient
t
instrumen
4
Nucleic Acid Applications
Measure using a Custom Factor
upper absorbance
)
sample
NanoDrop One User Guide
49
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