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4
Nucleic Acid Applications
Measure using a Custom Factor
46
NanoDrop One User Guide
2. Enter the
factor
to be used for the calculations and specify a
if desired.
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or
insert the blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to
instrument light path.
4. Select Blank and wait for the measurement to complete.
Tip: If
Auto-Blank
lower the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the
blanking cuvette.
6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm, or insert the
sample cuvette into the cuvette holder.
7. Start the sample measurement:
–
Pedestal: If
Auto-Measure
and tap Measure.
–
Cuvette: Tap Measure.
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
Typical nucleic acid spectrum
align the cuvette light path
is On, the blank measurement starts automatically after you
is On, lower arm; if Auto-Measure is off, lower arm
baseline correction
with the
Thermo Scientific
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