Thermo Scientific NanoDrop One User Manual page 123

Thermo Scientific
– lower arm to start measurement (or tap Measure if Auto-Measure is off)
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
– repeat substeps above for each additional standard (when specified number
of standards and replicates have been measured, a message asks whether
to load more standards or begin measuring samples)
– if finished measuring standards, tap Done (swipe left to view standard curve)
6. Measure samples:
– pipette 2 µL sample 1 onto pedestal, or insert sample 1 cuvette
– lower arm to start measurement (or tap Measure if Auto-Measure is off)
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
7. When you are finished measuring samples, tap End Experiment.
8. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
Related Topics
• Working with standard curves
• Best practices for protein measurements
• Measure a Micro-Volume Sample
• Measure a Sample Using a Cuvette
• Prepare Samples and Blanks
• Basic Instrument Operations
5
Protein Applications
Measure Protein Lowry
NanoDrop One User Guide 121