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Best practices for micro-volume measurements
Cleaning pedestals for daily
operation
• Before first measurement, clean
both pedestals with a new
laboratory wipe.
•
Run a blanking cycle
pedestals are clean.
• After each measurement, clean
both pedestals with new wipe to
prevent carryover.
• After each set of measurements,
clean pedestals with DI H2O (see
Clean pedestals between
•
Recondition pedestals
to maintain their hydrophobic
property.
Pipetting Samples
• Use
recommended sample
volumes
to ensure proper liquid
column formation.
• Use calibrated precision pipettor
(0–2 µL volume range) with
well-fitting, low-retention precision
tips to apply sample material to
instrument for measurement.
If using low accuracy (0-10 µL)
pipettor, use 2 µL sample volumes.
• Use new tip for each blank and
sample aliquot.
• Use new aliquot of sample for each
measurement.
• If solvents are used, make sure
they are compatible with the
pedestals. (see "Compatible
Solvents" in
Hazardous
Thermo Scientific
to verify
users)
periodically
Materials).
9
Learning Center
Measure a Micro-Volume Sample
NanoDrop One User Guide
197
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