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Protein Pierce 660 standard curve screen
Thermo Scientific
Note
• A baseline correction is performed at 750 nm (absorbance value at 750 nm is
subtracted from absorbance values at all wavelengths in sample spectrum).
• Micro-volume absorbance measurements and measurements taken with
nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.
The standard curve screen shows graphically the relationship between the
measured standards, the calculated standard curve, and the measured absorbance
and calculated concentration for a selected sample. A horizontal line connects the
sample absorbance value on the Y-axis to the standard curve. A vertical line
connects that point to the sample concentration value on the X-axis.
2
The R
value indicates how well the standard curve fits the standard data points (1.0
is a perfect fit; that is, all points lie exactly on the curve).
5
Protein Applications
Measure Protein Pierce 660
NanoDrop One User Guide
131
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