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5
Protein Applications
Measure Proteins and Labels
94
NanoDrop One User Guide
Related Topics
• Beer-Lambert Equation
• Protein A280 Calculations
Sample Pathlength
• For micro-volume measurements, the software selects
the optimal pathlength (between 1.0 mm and 0.03 mm)
based on sample absorbance at the analysis wavelength.
• For cuvette measurements, pathlength is determined by
the cuvette Pathlength setting in the software (see
General
Settings).
• Displayed spectra and absorbance values are normalized
to a 10 mm pathlength equivalent.
Reported Values
• Protein concentration. Reported in selected unit
(mg/mL or µg/mL). Calculations are based on
Beer-Lambert equation using corrected protein
absorbance value.
• Dye1/Dye2 concentration. Reported in pmol/µL.
Calculations are based on Beer's Law equation using
(sloping) baseline-corrected dye absorbance value(s).
Thermo Scientific
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