Measure Protein Lowry; Measure Total Protein Concentration - Thermo Scientific NanoDrop One User Manual

Measure Protein Lowry

Measure Total Protein Concentration

Theory of Protein Lowry assay
Protein assay kits and protocols
Thermo Scientific
Measures total protein concentration of
unpurified protein samples using a
Folin-Ciocalteu colorimetric detection
reagent.
Measure Total Protein
Reported Results
Settings
Detection Limits
The Protein Lowry assay uses Folin-Ciocalteu as a colorimetric detection reagent to
determine total protein concentration in unpurified protein samples. This application
is an alternative to the other colorimetric applications for measuring dilute protein
solutions or proteins in the presence of components that exhibit significant
absorbance between 200 nm and 280 nm. This application measures absorbance
at 650 nm and uses a standard curve to calculate protein concentration. See
Working with Standard Curves
correction is applied.
The Protein Lowry assay involves the reaction of protein with cupric sulfate in alkaline
solution, resulting in the formation of tetradentate copper-protein complexes. The
Folin-Ciocalteu reagent is effectively reduced in proportion to the chelated
copper-complexes. The water-soluble blue reaction product is measured at 650 nm
and baseline-corrected using the absorbance value at 405 nm. Pre-formulated kits
of Folin-Ciocalteu reagent and CuSO
Follow the assay kit manufacturer's recommendations for all standards and samples
(unknowns). Ensure each is subjected to the same timing and temperature
throughout the assay.
for more information. A single-point baseline
are available from us or a local distributor.
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NanoDrop One User Guide
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Protein Applications
Measure Protein Lowry
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