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Reported Values
• Nucleic acid concentration. Reported in selected
unit (i.e., ng/µL, µg/uL or µg/mL). Calculations are based
on modified Beer's Law equation using corrected nucleic
acid absorbance value.
• A260/A280 purity ratio. Ratio of corrected
absorbance at 260 nm to corrected absorbance at
280 nm. An A260/A280 purity ratio of ~1.8 is generally
accepted as "pure" for DNA (~2.0 for RNA). Acidic
solutions may under represent the reported value by
0.2-0.3; the opposite is true for basic solutions.
• A260/A230 purity ratio. Ratio of corrected
absorbance at 260 nm to corrected absorbance at
230 nm. An A260/A230 purity ratio between 1.8 and 2.2
is generally accepted as "pure" for DNA and RNA.
Note: Although purity ratios are important indicators of
sample quality, the best quality indicator quality is
functionality in the downstream application of interest (e.g.,
real-time PCR).
• Factor. Used in conjunction with Beer's Law to calculate
sample concentration
• Contaminant - If a contaminant was identified by the
Acclaro software, the contaminant will be displayed in this
column.
• A260 absorbance.
• A280 absorbance.
• Baseline correction.
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