Thermo Scientific NanoDrop One User Manual page 210

9 Learning Center
Prepare Samples and Blanks
1. From the Home screen, select an
application name.
2. Lift the instrument arm and clean the
upper and lower pedestals with new
laboratory wipe.
3. Measure a water blank:
– Pipette exactly 1 µL deionized
water (DI H O) onto the lower
2
pedestal and lower the arm.
– Tap Blank and wait for the
measurement to complete.
– Lift the arm and clean both
pedestals with new laboratory
wipe.
4. Measure the buffer solution:
– Pipette 1-2 µL buffer solution onto
the pedestal and lower the arm.
– Start the sample measurement:
– if Auto-Measure
arm
– if Auto-Measure is off, lower
arm and tap Measure
– Wait for measurement to complete.
The resulting spectrum should vary no
more than 0.04 A from the baseline at
the analysis wavelength.
If your spectrum does not meet these
criteria, repeat steps 2–4.
If spectrum is still outside
specifications, see
Blanking Problems.
5. When you are finished with the
blanking cycle, tap
End Experiment.
6. Lift the arm and clean both pedestals
with a new wipe.
208 NanoDrop One User Guide
is On, lower
Solutions for
Example spectrum of buffer suitable
for Protein A280 protein quantification
Example spectrum of buffer unsuitable
for Protein A280 protein quantification
Thermo Scientific