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Choosing and Measuring a Blank
For best results:
• For most applications, blank with the same
buffer solution used to resuspend the analyte
of interest. The blanking solution should be a
similar pH and ionic strength as the analyte
solution. For details, see "To measure
samples" in the application used.
• Measure new blank before each set of
samples. It is not necessary to blank the
instrument before each sample
measurement unless the samples are
dissolved in different buffer solutions.
• Measure a new blank every 30 minutes.
• Run a
blanking cycle
of your blanking solution before using it to
perform sample measurements. For a quick
demonstration, watch the multimedia training
Evaluating a Blanking Solution for
The resulting spectrum should vary no more
than 0.04 A (10 mm equivalent) across the
spectrum, especially at the analysis
wavelength as in the example at the right.
If the resulting spectrum is greater than
0.04 A around the analysis wavelength, that
buffer solution may interfere with the sample
analyses, especially for low concentration
samples. See below for details.
Thermo Scientific
The buffer used to resuspend a sample analyte can contribute absorbance. Blanking
minimizes any absorbance contribution due to the buffer components from the
sample measurement. The resulting sample spectrum represents the absorbance of
only the analyte of interest. For more information, watch the multimedia training
is a blank?
to assess the suitability
Suitability.
Good blanking buffer (measured abs < 0.04)
9
Learning Center
Prepare Samples and Blanks
NanoDrop One User Guide
What
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