To Measure Od600 Samples - Thermo Scientific NanoDrop One User Manual

To measure OD600 samples

Thermo Scientific
– Measure a series of dilutions using a young overnight culture (~16 hrs) of the
microbial strain
– Graph the OD600 measurements against the dilution factor
The upper detection limit is the measured OD600 value at which there ceases to
be a linear correlation between dilution factors and OD600 readings.
• Mix samples gently but thoroughly immediately before taking an aliquot for
measurement.
• For micro-volume measurements:
– Ensure pedestal surfaces are properly
– Avoid introducing bubbles when mixing and pipetting.
– Start the measurement promptly to avoid settling or evaporation.
– Follow best practices for micro-volume measurements.
– Use 2 µL sample volume. See
information.
– For dilute samples that exhibit low absorbance at 600 nm, use an alternative
wavelength such as 400 nm to measure absorbance, or use cuvettes
instead of micro-volume measurements.
• For cuvette measurements (NanoDrop One
– Use clean plastic, glass or quartz cuvettes.
– Follow best practices for cuvette measurements.
– Do not use the automatic
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
cleaned and
Recommended Sample Volumes
C
instruments only):
stirring feature for this assay.
NanoDrop One User Guide
6
Measure OD600
conditioned.
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