Thermo Scientific NanoDrop One User Manual page 40

4 Nucleic Acid Applications
Measure Microarray
Setting Available Options
Oligo RNA with non-editable
calculated factor in ng-cm/µL
Custom (with user-specified factor in
ng-cm/µL)
Dye 1/Dye 2 Cy3, 5, 3.5, or 5.5,
a
Type Alexa Fluor 488, 546, 555, 594, 647,
or 660
Dye 1/Dye 2 Unit picomoles/microliter (pmol/uL),
micromoles (uM), or millimoles (mM)
Analysis On or off
b
Correction
Enter analysis correction wavelength
in nm or use default value (340 nm)
a
To add a custom dye or edit the list of available dyes, use the Dye/Chromophore Editor.
b
The Analysis Correction affects the calculation for nucleic acid concentration only.
Dye/chromophore editor
38 NanoDrop One User Guide
Use the Dye/Chromophore Editor to add a custom dye to the list of available dyes in
Microarray Setup or Proteins & Labels
available in that list.
To access the Dye/Chromophore Editor:
• from the Home screen, tap
• from the Microarray or Proteins & Labels measurement screen, tap
Settings > Dye Editor
Description
Factor calculated from user-defined RNA base
sequence. When selected, available RNA base
units (i.e., G, A, U, C) appear as keys. Define
sequence by tapping appropriate keys. Factor
is calculated automatically based on widely
accepted value for each base unit.
Enter factor between 15 ng-cm/µL and
150 ng-cm/µL
Select pre-defined dye(s) used to label sample
material, or one that has been added using Dye
Editor.
Select unit for reporting dye concentrations
Corrects sample absorbance measurement for
any offset caused by light scattering particulates
by subtracting absorbance value at specified
analysis correction wavelength from
absorbance value at analysis wavelength.
Corrected value is used to calculate sample
concentration.
Tip: If the sample has a modification that
absorbs light at 340 nm, select a different
correction wavelength or turn off Analysis
Correction.
Setup. You can also specify which dyes are
> Dye Editor
>
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