Thermo Scientific NanoDrop One User Manual page 140

6 Measure OD600
138 NanoDrop One User Guide
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure an OD600 sample
1. From the Home screen, select the OD600 tab and tap OD600.
2. Specify the cell number conversion factor
or absorbance correction
3. Pipette 2 µL blanking solution (i.e., the media solution the cells of interest are
suspended in) onto the lower pedestal and lower the arm, or insert the blanking
cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to
instrument light path.
4. Tap Blank and wait for the measurement to complete.
Tip: If Auto-Blank
lower the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove
the blanking cuvette.
6. Pipette 2 µL sample solution onto the pedestal and lower the arm, or insert the
sample cuvette into the cuvette holder.
7. Start the sample measurement:
– Pedestal: If Auto-Measure
arm and tap Measure.
– Cuvette: Tap Measure
When the sample measurement is completed, the spectrum and reported
values are displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
Related Topics
• Measure a Micro-Volume Sample
• Measure a Sample Using a Cuvette
if desired.
align the cuvette light path
is On, the blank measurement starts automatically after you
is On, lower arm; if Auto-Measure is off, lower
Cleaning the
and a second monitored wavelength
with the
Thermo Scientific