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9
Learning Center
Micro-Volume Sampling—How it Works
Micro-Volume Sampling—How it Works
Surface Tension
Absorbance Spectrum
Sample Absorbance
Sample Concentration
Baseline Correction
184
NanoDrop One User Guide
Surface Tension
C
The NanoDrop One
spectrophotometer uses
surface tension to hold a small volume of sample
between two pedestals. The patented sample
retention system enables the measurement of highly
concentrated samples without the need for dilutions.
A fiber optic cable embedded in the upper pedestal
leads to a xenon light source. A second cable
embedded in the lower pedestal leads to a detector.
When the instrument arm is down, the sample forms
a liquid column, essentially bridging the gap between
the two fiber optic cables.
Absorbance Spectrum
The light passes through the liquid column to the
detector, which generates a spectrum of absorbance
versus wavelength. The spectrum shows the amount
of light absorbed by the molecules of the sample at
each measured wavelength.
Note: To prevent evaporation, which affects
measurement accuracy, close the arm quickly after
you finish loading a sample or blank.
The example at the left shows a typical absorbance
spectrum taken of a nucleic acid sample. The
spectrum is measured from 190 nm to 850 nm. The
displayed range may vary for each application.
Thermo Scientific
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