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Setting
Available Options
Baseline
On or off
Correction
Enter baseline
correction
wavelength in nm or
use default value
(340 nm)
Related Topics
•
Detection Limits for Oligo DNA and Oligo RNA Measurements
The lower detection limits and reproducibility specifications for the oligonucleotide
sample types (ssDNA and RNA) are provided here. The upper detection limits are
dependent on the
coefficients for the user-defined
To calculate upper detection limits for nucleic acid samples
To calculate upper detection limits in ng/µL, use the following equation:
For example, for a sample measurement using an extinction coefficient of 55, the
equation looks like this:
Thermo Scientific
Description
• Molecular Weight of oligo calculated from user-defined
base sequence.
• Number of Bases entered.
• Molar Ext. Coefficient (260 nm). Molar extinction
coefficient of oligo (in ng-cm/µL) at 260 nm calculated from
entered base sequence.
• %GC. Percentage of guanine and cytosine residues in
total number of bases entered.
Corrects for any offset caused by light scattering particulates
by subtracting measured absorbance at specified baseline
correction wavelength from absorbance values at all
wavelengths in sample spectrum. As a result, absorbance of
sample spectrum is zero at specified baseline correction
wavelength.
Tip: If the sample has a modification that absorbs light at
340 nm, select a different correction wavelength or turn off
Baseline Correction.
Instrument Settings
upper absorbance limit
(upper absorbance limit
of the instrument and the extinction
base sequences.
* extinction coefficient
t
instrumen
NanoDrop One User Guide
4
Nucleic Acid Applications
Measure Oligo DNA or Oligo RNA
)
sample
57
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