Thermo Scientific NanoDrop One User Manual page 117

Micro-uv/vis spectrophotometer
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Menu of options;
tap to open
Standard curve
Red squares indicate sample
data points
Pinch and zoom to adjust axes;
double-tap to reset
Protein Bradford reported values
Thermo Scientific
Curve type
Selected sample
Note
• A baseline correction is performed at 750 nm (absorbance value at 750 nm is
subtracted from absorbance values at all wavelengths in sample spectrum).
• Micro-volume absorbance measurements and measurements taken with
nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.
The initial screen that appears after each measurement and the standards screen
(see previous image) show a summary of the reported values. To view all reported
values, press and hold the sample row. Here is an example:
2
R
value (1.0 equals
perfect fit)
White circles indicate
standard data points
Page control; swipe screen left or right
to view next or previous screen
5
Protein Applications
Measure Protein Bradford
Protein concentration for
selected sample
Absorbance
at 595 nm
Tap row to
select
sample and
update
graph; tap
more rows
to overlay
abs/conc
lines for up
to five
samples.
Press and
hold
sample row
for
measureme
nt details.
Drag tab down/up
to see more/less
sample data
NanoDrop One User Guide
115

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