Measure Protein Bca; Measure Total Protein Concentration - Thermo Scientific NanoDrop One User Manual

Measure Protein BCA

Measure Total Protein Concentration

Theory of Protein BCA assay
Protein assay kits and protocols
Thermo Scientific
Measures total protein concentration of
unpurified protein samples using a
bicinchoninic acid colorimetric detection
reagent.
Measure Total Protein
Reported Results
Settings
Detection Limits
The Protein BCA assay uses bicinchoninic acid as a colorimetric detection reagent
to determine total protein concentration in unpurified protein samples. This
application is useful for measuring dilute protein solutions or proteins in the presence
of components that exhibit significant absorbance between 200 nm and 280 nm,
which rules out direct protein measurements at 280 nm or 205 nm. This application
measures absorbance at 562 nm and uses a standard curve to calculate protein
concentration. A single-point baseline correction is applied.
The Protein BCA assay uses bicinchoninic acid (BCA) as the detection reagent for
+1
Cu , which is formed when Cu
environment. A purple reaction product is formed by the chelation of two molecules
of BCA with one cuprous ion (Cu
presence of protein is measured at 562 nm and baseline-corrected using the
absorbance value at 750 nm. Pre-formulated kits of BCA reagent and CuSO
available from us or a local distributor.
Please refer to the NanoDrop website for up-to-date kits and protocols for the
NanoDrop One instruments. Follow the assay kit manufacturer's recommendations
for all standards and samples (unknowns). Ensure each is subjected to the same
timing and temperature throughout the assay.
+2
is reduced by certain proteins in an alkaline
+1
). The resulting Cu-BCA chelate formed in the
NanoDrop One User Guide
5
Protein Applications
Measure Protein BCA
are
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