Thermo Scientific NanoDrop One User Manual page 113

Thermo Scientific
3. Measure blank:
– pipette 2 µL DI H
blanking cuvette into cuvette holder
Tip: If using a cuvette, make sure to
light path.
– tap Blank and wait for measurement to complete
– lift arm and clean both pedestals with new laboratory wipe, or remove
cuvette
4. Measure reference standard:
– pipette 2 µL reference solution onto pedestal, or insert reference cuvette
(reference solution should contain none of the standard protein stock, see
Working With Standard Curves
– lower arm to start measurement (or tap Measure if Auto-Measure is off)
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
5. Measure remaining standards:
– pipette 2 µL standard 1 onto pedestal, or insert standard 1 cuvette
– lower arm to start measurement (or tap Measure if Auto-Measure is off)
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
– repeat substeps above for each additional standard (when specified number
of standards and replicates have been measured, a message asks whether
to load more standards or begin measuring samples)
– if finished measuring standards, tap Done (swipe left to view standard curve)
6. Measure samples:
– pipette 2 µL sample 1 onto pedestal, or insert sample 1 cuvette
– lower arm to start measurement (or tap Measure if Auto-Measure is off)
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
7. When you are finished measuring samples, tap End Experiment.
8. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
O onto lower pedestal and lower arm, or insert DI H
2
align cuvette light path
for details)
5
Protein Applications
Measure Protein Bradford
with instrument
NanoDrop One User Guide
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