Thermo Scientific NanoDrop One User Manual page 165

Setting
Standards (Standard
curve methods only)
Thermo Scientific
Available Options
Notes:
• Refer to product literature for information about factors and extinction
coefficients for specific materials.
• To set up a method that reports absorbance measurements only, select
Factor or Extinction Coefficient with the factor or extinction coefficient set
to "1".
• If specified unit for factor or extinction coefficient is based on mass (such
as mg/mL) and specified unit for calculated result is based on molarity
(such as pmol/µL) or vice versa, enter molecular weight and use
adjacent drop down list to select appropriate unit.
Define the standards:
• Enter name and analyte concentration of each standard and a reference,
if desired:
– Depending on the Curve Type setting, a standard curve can be
generated using two or more standards. (The software allows a
reference and up to 7 standards.)
– All reference and standards solutions should be in the same
buffer used to resuspend the samples plus the same volume of
reagent added to the samples.
– First standard can be a reference measurement. The reference
solution should contain none of the analyte of interest. (The reference
measurement is not the same as a blank measurement.)
– Concentration values for standards can be entered in any
order but the standards must be measured in the order in which they
were entered; however, best practice dictates that standards be
measured from the lowest concentration of the standard analyte
stock to the highest.
– Concentration range of the standards must cover the
dynamic range of the assay and the expected range of the unknown
samples. Sample analyte concentrations are not extrapolated beyond
the concentration of the highest standard.
7
Custom Applications
Measure Custom
NanoDrop One User Guide 163