Settings For Kinetic Measurements - Thermo Scientific NanoDrop One User Manual

8 Measure Kinetics

Settings for Kinetic Measurements

Tab
Name and Range
Stages and Intervals
180 NanoDrop One User Guide
To show the Kinetics settings, from the instrument Home screen, tap Kinetics (tab)
> Kinetics (Method), and either tap Create Method or select a method and tap
Edit Method. You can also display the settings from any Kinetics measurement
screen, by tapping
The settings appear on two tabs: "Name and Range" and "Stages and Intervals."
See the table below for details.
Setting
Method name
Description
Measurement range
Monitored
wavelengths
Number of Stages
Time Unit
> Kinetics Setup.
Description
Enter a name for this method (this name appears in
the Kinetics Setup box after the method has been
saved).
Enter a detailed description for this method, if
desired, such as the type of samples, added reagents,
etc.
Select the spectral range in which this method will
acquire data. Available options:
• Ultra-violet only (190 nm - 350 nm)
• Visible only (350 nm - 850 nm)
• Ultra-violet and visible (190 nm - 850 nm)
• Custom (specify starting and ending point in
nanometers)
Note: For measurements taken with nonstandard
(other than 10 mm) cuvettes, the spectra are normalized
to a 10 mm pathlength equivalent.
Enter up to 3 wavelengths to be measured and
reported at run time.
Note: All specified wavelengths must fall within the
selected measurement
Specify up to 5 stages for kinetic measurements.
Each stage can have unique Delay, Interval Time and
# Intervals settings.
Note: Many kinetic measurements include only one
stage. Additional stages are necessary only when a
variation in stage interval or duration is needed.
Select the unit for time-based measurements (seconds
or minutes).
range.
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