Recommended Sample Volumes - Thermo Scientific NanoDrop One User Manual

9 Learning Center
Measure a Micro-Volume Sample

Recommended sample volumes

198 NanoDrop One User Guide
Application
Nucleic acid (aqueous solution)
Purified protein
Other protein applications such as Bradford or BCA
Microbial cell suspensions
a
Use 2 µL for samples that contain materials that may reduce surface tension such as a surfactant.
To measure a micro-volume sample
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
1. From the instrument Home screen, select an
application from one of the application categories,
such as UV-Vis, or Custom Methods.
2. Lift the instrument arm and clean the upper and lower
pedestals with new laboratory wipe.
Sample Volume
a
1 µL
2 µL
2 µL
2 µL
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