Thermo Scientific NanoDrop One User Manual page 86

5 Protein Applications
Measure Protein A205
84 NanoDrop One User Guide
Sample Pathlength
• For micro-volume measurements, the software selects
the optimal pathlength (between 1.0 mm and 0.03 mm)
based on sample absorbance at the analysis wavelength.
• For cuvette measurements, pathlength is determined by
the cuvette Pathlength setting in the software (see
General Settings).
• Displayed spectra and absorbance values are normalized
to a 10 mm pathlength equivalent.
Reported Values
• Protein concentration. Reported in selected unit
(mg/mL or µg/mL). Calculations are based on
Beer-Lambert equation using corrected protein
absorbance value.
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