Thermo Scientific NanoDrop One User Manual page 42

4 Nucleic Acid Applications
Measure Microarray
40 NanoDrop One User Guide
– select default Unit that will be used to display dye concentration
– enter dye's Extinction Coefficient (or molar absorptivity constant) in
L/mole-cm (typically provided by dye manufacturer)
– specify Wavelength in nm (between 450 nm and 700 nm) that will be used
to measure dye's absorbance
– specify dye's correction values at 260 nm and 280 nm
– tap Add Dye
Note To determine dye correction values (if not available from dye
manufacturer):
– use instrument to measure pure dye and note absorbance at 260 nm,
280 nm and at analysis wavelength for dye (see above)
– calculate ratio of A
Correction
– calculate ratio of A
Correction
When a custom dye is selected before a measurement, the dye's absorbance
and concentration values are reported and the corrections are applied to the
measured sample absorbance values, and to the resulting sample
concentrations and purity ratios.
Edit custom dye
Tip Dyes pre-defined in the software cannot be edited.
– tap to select custom dye
– tap
– edit any entries or settings
– tap Save Dye
Delete custom dye
Tip Dyes pre-defined in the software cannot be deleted.
– tap to select custom dye
– tap
NOTICE Deleting a custom dye permanently removes the dye and all
associated information from the software.
/A and enter that value for 260 nm
260 dye wavelength
/A and enter that value for 280 nm
280 dye wavelength
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