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Tab
Thermo Scientific
Setting
Stage 1, 2, etc.
Description
Specify the available settings for each stage:
• Delay. Specify a delay before a stage starts.
• Interval Time. Specify the length of time between
measurements taken during this stage (minimum is
2 seconds). The first measurement occurs when the
stage starts (or after the delay is completed if a
Delay is specified).
Note: If two or more stages are specified with Delay
set to zero, two measurements occur at the same
time (the measurement at the beginning of the new
stage directly overlaps the one at the end of the
previous stage).
• # Intervals. Specify the number of absorbance
measurements to take in this stage.
Note: Since the first measurement is taken when
the stage starts, the number of measurements
reported for each stage will be the # Intervals
setting plus 1.
• Duration. Readout shows the total time required
for this stage, including any delay and all specified
intervals.
The colored rows at the right (see image below) show
the start and end times for each stage; the colored
boxes at the right correspond with the specified delay
and number of intervals for each stage.
If no delay is specified, absorbance measurements are
taken at the start and end of each stage and after each
specified interval. If a delay is specified, as in stage 3
above, the first measurement occurs at the start of the
first interval. If the unit is seconds in the example above,
a total of 11 measurements are taken at the following
times over a period of 32 seconds:
8
Measure Kinetics
NanoDrop One User Guide
181
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