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Detection limits for pre-defined dyes
Sample Type
Cy3, Cy3.5, Alexa
Fluor 555, Alexa Fluor
660
Cy5, Cy5.5, Alexa
Fluor 647
Alexa Fluor 488, Alexa
Fluor 594
Alexa Fluor 546
a
Values are approximate
b
Based on five replicates (SD=ng/µL; CV=%)
Thermo Scientific
Note To minimize instrument error with highly concentrated samples, make
dilutions to ensure that measurements are made within these absorbance limits:
• For micro-volume measurements, maximum absorbance at 260 nm (for
nucleic acids) or 280 nm (for proteins) should be less than 62.5 A.
• For measurements with 10 mm pathlength cuvettes, maximum absorbance
at 260 nm (or 280 nm for proteins) should be less than 1.5 A, which is
approximately 75 ng/µL dsDNA.
Lower Detection
Limit
0.2 pmol/µL
(pedestal)
0.12 pmol/µL
(pedestal)
0.4 pmol/µL
(pedestal)
0.3 pmol/µL
(pedestal)
3
Upper Detection
a
Limit
100 pmol/µL
(pedestal)
60 pmol/µL
(pedestal)
215 pmol/µL
(pedestal)
145 pmol/µL
(pedestal)
Application Measurement Ranges
Detection Limits for All Applications
Typical Reproducibility
±0.20 pmol/µL for sample
concentrations between 0.20 and
4.0 pmol/µL;
±2% for samples >4.0 pmol/µL
±0.12 pmol/µL for sample
concentrations between 0.12 and
2.4 pmol/µL;
±2% for samples >2.4 pmol/µL
±0.40 pmol/µL for sample
concentrations between 0.40 and
8.0 pmol/µL;
±2% for samples >8.0 pmol/µL
±0.30 pmol/µL for sample
concentrations between 0.30 and
6.0 pmol/µL;
±2% for samples >6.0 pmol/µL
NanoDrop One User Guide
b
21
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