Best Practices For Cuvette Measurements - Thermo Scientific NanoDrop One User Manual

9 Learning Center
Measure a Sample Using a Cuvette

Best practices for cuvette measurements

• The instrument arm can be up or
down for cuvette measurements.
• Use 10 mm, 5 mm, 2 mm or 1 mm
cuvettes.
• Clean and dry cuvette after each
measurement.
• Use cuvettes that are free of
scratches and avoid fingerprints
which may affect results.
• Use quartz cuvettes or UV-grade
plastic cuvettes to measure
samples with analysis wavelengths
in the UV range (<340 nm).
• Micro, semi-micro, and ultra-micro
cuvettes should be masked.
• Fill cuvettes with enough blanking
or sample solution to cover
instrument optical path (2 mm
sample beam is 8.5 mm above
cuvette bottom).
• Lift instrument arm and make sure
cuvette holder is free of debris.
• When inserting quartz or masked
plastic cuvettes, align cuvette light
path with instrument light path.
202 NanoDrop One User Guide
To measure a sample using a cuvette
NOTICE
• To prevent damage from spills, keep containers of liquids away from the
instrument.
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument ans may cause permanent damage.
Instrument light path
Cuvette holder
Thermo Scientific