Thermo Scientific NanoDrop One User Manual page 81

Micro-uv/vis spectrophotometer
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To measure a Protein A205 sample
1. From the Home screen, from the Proteins tab, select Protein A205.
2. Specify a
sample type
3. Pipette 1–2 µL of the blanking solution onto the lower pedestal and lower the
arm, or insert the blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to
instrument light path.
4. Tap Blank and wait for the measurement to complete.
Tip: If
Auto-Blank
lower the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove
the blanking cuvette.
6. Pipette 2 µL sample solution onto the pedestal and lower the arm, or insert the
sample cuvette into the cuvette holder.
7. Start the sample measurement:
Pedestal: If
Auto-Measure
arm and tap Measure.
Cuvette: Tap Measure
When the sample measurement is completed, the spectrum and reported
values are displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
and
baseline correction
align the cuvette light path
is On, the blank measurement starts automatically after you
is On, lower arm; if Auto-Measure is off, lower
5
Protein Applications
Measure Protein A205
if desired.
with the
NanoDrop One User Guide
79

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