Settings For Nucleic Acid Measurements Using A Custom Factor - Thermo Scientific NanoDrop One User Manual

4 Nucleic Acid Applications
Measure using a Custom Factor

Settings for Nucleic Acid Measurements using a Custom Factor

Setting
Custom Factor
Baseline Correction
48 NanoDrop One User Guide
Related Topics
• Basic Instrument Operations
• Nucleic Acid Reported Results
• Nucleic Acid Calculations
To show the Custom Factor settings, from the local control, tap
Factor Setup.
When using the PC Control software, from the Custom Factor measurement screen,
select the settings icon
Available Options
Enter an integer value
between 15 ng-cm/µL
and 150 ng-cm/µL
On or off
Enter baseline
correction wavelength
in nm or use default
value (340 nm)
Related Topics
• Instrument Settings
to view the Custom Factor Setup.
Description
Constant used to calculate nucleic acid concentration in
modified Beer's Law equation. Based on extinction
coefficient and pathlength:

f = 1/( * b))
260
where:

f= factor
= molar extinction coefficient at 260 nm in ng-cm/µL
b = sample pathlength
measured with the NanoDrop One instruments)
Optional user-defined baseline correction. Can
be used to correct for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from absorbance
values at all wavelengths in sample spectrum. As a result,
absorbance of sample spectrum is zero at specified
baseline correction wavelength.
NOTE: Baseline correction is selected from the
measurement screen of the PC control software and is not
shown in the Custom Factor Setup.
> Custom
in cm (1 cm for nucleic acids
Thermo Scientific