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4
Nucleic Acid Applications
Measure Microarray
44
NanoDrop One User Guide
Related Topics
•
Calculations for Nucleic Acid Measurements
Reported Values
• Nucleic acid concentration. Reported in selected
unit (i.e., ng/µL, µg/uL or µg/mL). Calculations are based
on modified Beer's Law equation using corrected nucleic
acid absorbance value.
• A260/A280 purity ratio. Ratio of corrected
absorbance at 260 nm to corrected absorbance at
280 nm. An A260/A280 purity ratio of ~1.8 is generally
accepted as "pure" for DNA (~2.0 for RNA). Acidic
solutions may under represent the reported value by
0.2-0.3; the opposite is true for basic solutions.
• Dye1/Dye2 concentration. Reported in pmol/µL.
Calculations are based on Beer's Law equation using
(sloping) baseline-corrected dye absorbance value(s).
Note: Although purity ratios are important indicators of
sample quality, the best indicator of DNA or RNA quality is
functionality in the downstream application of interest (e.g.,
microarray).
Thermo Scientific
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