To Measure Protein Lowry Standards And Samples - Thermo Scientific NanoDrop One User Manual

5 Protein Applications
Measure Protein Lowry

To measure Protein Lowry standards and samples

120 NanoDrop One User Guide
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure Protein Lowry standards and samples
1. From the Home screen, select the Proteins tab and tap Protein Lowry.
2. Specify a curve type
concentration of each
Tip: For this assay, we recommend setting Curve Type to "2nd Order
Polynomial."
3. Measure blank:
– pipette 2 µL DI H
blanking cuvette into cuvette holder
Tip: If using a cuvette, make sure to
light path.
– tap Blank and wait for measurement to complete
– lift arm and clean both pedestals with new laboratory wipe, or remove
cuvette
4. Measure reference standard:
– pipette 2 µL reference solution onto pedestal, or insert reference cuvette
(reference solution should contain none of the standard protein stock, see
Working With Standard Curves
– lower arm to start measurement (or tap Measure if Auto-Measure is off)
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
5. Measure remaining standards:
– pipette 2 µL standard 1 onto pedestal, or insert standard 1 cuvette
and number of replicates for each standard
standard.
O onto lower pedestal and lower arm, or insert DI H
2
align cuvette light path
for details)
Cleaning the
and enter the
O
2
with instrument
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