Thermo Scientific NanoDrop One User Manual page 28

4 Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
26 NanoDrop One User Guide
• Ensure the sample absorbance is within the instrument's
limits.
• Blank with the same buffer solution used to resuspend the analyte of interest.
The blanking solution should be a similar pH and ionic strength as the analyte
solution.
• Run a blanking cycle
solution. If the buffer exhibits strong absorbance at or near the analysis
wavelength (typically 260 nm), you may need to choose a different buffer or
application. See Choosing and Measuring a Blank
• For micro-volume measurements:
– Ensure pedestal surfaces are properly
– If possible, heat highly concentrated or large molecule samples, such as
genomic or lambda DNA, to 63 °C (145 °F) and gently (but thoroughly) vortex
before taking a measurement. Avoid introducing bubbles when mixing and
pipetting.
– Follow best practices for micro-volume measurements.
– Use a 1-2 µL sample volume. See
information.
• For cuvette measurements (NanoDrop One
cuvettes and follow
Related Topics
• Measure a Micro-Volume Sample
• Measure a Sample Using a Cuvette
• Best Practices for Micro-Volume Measurements
• Best Practices for Cuvette Measurements
• Prepare Samples and Blanks
• Basic Instrument Operations
to assess the absorbance contribution of your buffer
Recommended Sample Volumes
best practices for cuvette measurements.
absorbance detection
for more information.
cleaned and conditioned.
C
instruments only), use compatible
for more
Thermo Scientific