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Sample Pathlength
Blank Button
Measure Button
Thermo Scientific
All applications display the sample pathlength along the spectrum's vertical axis.
Micro-volume absorbance measurements and measurements taken with
nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent. Here is an
example.
Sample
Tap Blank to measure a blank for the selected experiment.
A blank must be measured before each group of similar samples. The blank solution
is typically the pure buffer that was used to resuspend the sample. For more
information, see
Choosing and Measuring a
Tap Measure to measure a sample for the selected experiment.
Samples must be properly isolated and prepared before they can be measured with
the instrument and the concentration must be within the instrument's absorbance
detection limits. For more information, see
Micro-Volume Sample
Limits.
Note The Measure button is enabled after a valid blank measurement is
completed.
Blank.
Preparing
or
Measure a Cuvette Sample
9
Learning Center
Basic Instrument Operations
Samples. and
Measure a
and
Absorbance Detection
NanoDrop One User Guide
215
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