Chapter 4 Nucleic Acid Applications; Measure Dsdna, Ssdna Or Rna - Thermo Scientific NanoDrop One User Manual

Micro-uv/vis spectrophotometer
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Nucleic Acid Applications

Measure dsDNA, ssDNA or RNA

Measure dsDNA, ssDNA or RNA
To measure dsDNA, ssDNA or RNA samples
Thermo Scientific
Measures the concentration of purified
dsDNA, ssDNA or RNA samples that
absorb at 260 nm.
Measure dsDNA, ssDNA or RNA
Reported Results
Settings
Detection Limits
Calculations
Use the dsDNA, ssDNA and RNA applications to quantify purified double-stranded
(ds) or single-stranded (ss) DNA or RNA samples. These applications report nucleic
acid concentration and two absorbance ratios (A260/A280 and A260/A230). A
single-point baseline correction can also be used.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
NanoDrop One User Guide
4
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