Thermo Scientific NanoDrop One User Manual page 136

Micro-uv/vis spectrophotometer
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5
Protein Applications
Measure Protein Pierce 660
Setting
Description
Curve Type
Specify type of equation used to create standard curve from standard concentration
values. Available options:
Replicates
Enter number of measurements of the reference or the same standard or sample
that are averaged together to produce its associated concentration value.
Note: Replicates setting cannot be changed after the first standard has been
measured.
Standards
Enter actual concentration value of each standard.
Note: Concentration values can be entered in any order but the standards must be
measured in the order in which they were entered.
If you also want to enter previously measured absorbance values for the standards,
select this check box:
and then enter absorbance values for all the standards.
134
NanoDrop One User Guide
Linear: Draws the linear least squares line through all measured standards
(requires reference measurement and at least one standard)
Interpolation: Draws a series of straight lines to connect all measured
standards (requires reference measurement and at least one standard)
nd
2
order polynomial: Draws the 2
using all measured standards (requires reference measurement and at least
two standards)
rd
3
order polynomial: Draws the 3
all measured standards (requires reference measurement and at least three
standards)
Related Topics
Instrument Settings
nd
order least squares polynomial
rd
order least squares polynomial using
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