Sample Introduction System Set-Up; Peristaltic Pump Tubing Options; Internal Standards - Thermo Scientific iCAP 6000 Series User Manual

Instrument Set-up

Sample Introduction System Set-up

When a sample is aspirated and a proportion of the aerosol generated by
Sample
the nebuliser is passed to plasma through the torch assembly, it
Introduction
generates a load on the plasma. The RF generator, the plasma
conditions and the sample introduction system should be optimised for
System Set-up
the particular sample and solvent type being introduced.
Peristaltic Pump
The iCAP 6000 requires pump tubing with two clamping points (two
bridges). Your instrument will be supplied with Tygon® tubing suitable
Tubing Options
for the aqueous installation tests.
A range of different tubing is available for different sample and matrix
types. For full details of these, refer to the Hardware Manual on the
Supplementary Information CD.
An internal standard is a reference element that can be used to correct

Internal Standards

for changes in signal intensity caused external factors. By definition it
should not occur naturally in the sample, but is added to compensate
for sampling differences. It must behave the same as other elements
requiring analysis in the sample.
Figure 2–16. Internal Standards Mixing Kit
The use of internal standards is not required for all types of analysis but
is typically employed where fluctuations in sample loading of the
plasma occur. This is often caused by differing sample physical
properties, for example viscosity, dissolved solids, surface tension or
volatility.
Rather than adding an internal standard manually to all the solutions to
be analysed it is possible to add it automatically on-line using the
Internal Standards Mixing Kit.
To minimise sample dilution, the tubing for the internal standard
solution has a smaller bore than that of the sample solution.
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Thermo Fisher Scientific iCAP User Guide